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Strand Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2
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Strand Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2 EMERGING INFECTIOUS DISEASES Hogan, C. A., Huang, C., Sahoo, M. K., Wang, H., Jiang, B., Sibai, M., Holubar, M., Mathew, R., Zehnder, J., Pinsky, B. A. 2021; 27 (2): 632–35Abstract
We developed an assay that detects minus-strand RNA as a surrogate for actively replicating severe acute respiratory syndrome coronavirus 2. We detected minus-strand RNA in 41 persons with coronavirus disease up to 30 days after symptom onset. This assay might inform clinical decision-making about patient infectiousness.
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